The binding legal principle established is that regulation 3(1)(b)(iv) of the Road Accident Fund Regulations, 2008 does not make the application of the AMA Guides in assessing whether a third party's injury is 'serious' dependent on the existence of operational guidelines published by the Minister of Transport. The words 'if any' in regulation 3(1)(b)(iv) qualify and apply to both 'operational guidelines' and 'amendments', rendering their publication discretionary. The obligation created by the word 'must' in the regulation is placed conditionally upon the medical practitioner to apply the AMA Guides in accordance with any operational guidelines or amendments if published, but does not create an obligation on the Minister to publish such guidelines. The publication of operational guidelines is not a condition precedent to the application of the AMA Guides. Medical practitioners can and must apply the AMA Guides in the absence of operational guidelines when assessing whether an injury qualifies as 'serious' for purposes of general damages claims under the Road Accident Fund Act.